Purification and enzymatic properties of a highly alkaline mannanase from alkaliphilic Bacillus sp. strain JAMB-750
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چکیده
An alkaliphilic Bacillus isolate (strain JAMB-750) was found to exoproduce a novel alkaline mannanase (AmA). AmA was purified to homogeneity (about 98% pure) as judged by SDS-polyacrylamide electrophoresis. The molecular mass was approximately 130 kDa. The hydrolysis patterns of various substrates indicated that the enzyme was an endo-type enzyme. The optimal temperature and pH for activity were 55C and around 10, respectively. The optimal pH is the highest among mannanases reported to date. Highly alkaline mannanase is expected to have good wash performance in detergent solution and be effective in biobleaching of kraft pulp. Therefore, AmA is highly applicable to the paper and detergent industries, as well as the food industry.
منابع مشابه
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